Regulations for Animal Use in Research

Preface:
Regulations pertaining to projects involving animals and the display of those projects reflect strict standards. While exhibitors' investigations of biological processes are to be encouraged, they are subject to the same laws, ethics, and regulations as any other individual researching this field. In the Animals for Research Act of Ontario, all vertebrates are afforded protection. Also, schools and science fairs are explicitly included in the definition of "research facility" in Ontario.
The regulations below are written in view of these laws. The display of a project is further restricted by Youth Science Canada in view of the need to maintain a positive public image towards science fairs. The restriction is due in part to a lack of essential expertise on the part of the student investigators and their immediate supervisors. There is also a desire to maximize the efficiency of animal use and to impress this on the students, especially regarding scientific merit.

Use of Invertebrate Animals in Research:
Definition
An invertebrate is an animal lacking a backbone, such as an arthropod, mollusc, annelid, insect, etc. Invertebrates comprise nearly 95% of animal species and about 30 different phyla. Invertebrates have small nervous systems, consisting of many small brains (ganglia).
Note: Cephalopods (squid, octopi) have large - vertebrate-like central nervous systems and are treated like vertebrates (see below).

Regulations
1. Exhibitors may do experiments on invertebrate animals.
2. WWSEF reserves the right to disallow any project involving invertebrate animals that is of questionable scientific or educational value, or is judged to be unethical.
Note: The Institute for Laboratory Animal Research (USA) has published a pamphlet listing 10 principles that define the ethical use of animals in a school or science fair setting.
Youth Science Canada and WWSEF endorse and promote these principles.

Use of Vertebrate Animals in Research:
Preface:
Definition
Animals with a backbone (vertebrate) or spinal column include mammals, birds, reptiles, amphibians and fishes. Cephalopods are invertebrates with large, vertebrate-like central nervous systems. This policy applies to vertebrates and cephalopods.
Biological experimentation is subject to legal restrictions including, among others:
o Criminal Code of Canada, Section 446, Cruelty to Animals;
o Health of Animals Act, Bill C-66;
o Canadian Council on Animal Care (CCAC)

Regulations
Before experimentation begins, Form 4.1C must be completed for every project that involves vertebrates or cephalopods, their embryos or their tissue, and submitted to the WWSEF Ethics Officer for approval.

1. Vertebrate animals (birds, fish, mammals, reptiles, amphibians), and cephalopods (squids, octopi) are not to be used in any active experiments, which may be deleterious to the health, comfort or physical integrity of the animals. This permits observation of wild animals, animals in zoological parks, farm animals and pets. Only animals acquired from biological supply houses may be used in "experiments". Animals from pet stores or from one's own breeding program cannot be used for these purposes.
2. Observation of pets, farm animals, animals in zoos and aquaria, and wild animals is permitted. Observation of wild animals falls within the definition of hunting in some jurisdictions. Students should obtain advice and permission from conservation authorities to ensure that they are not interfering with the animal's life, and to ensure that their project is permissible. A permit may be required.
3. Behavioural experiments with positive rewards are permissible only if the animal is not placed in a stress situation. Training an animal to travel through a maze to receive a food reward is stressful, particularly if the animal is hungry, and is therefore not permissible. However, allowing an animal to make a free choice (of food, for example) is permissible, so long as the animal is not stressed before offering the choice (e.g. by withholding food).
4. Studies of embryos are similarly restricted to observation, without intervention with drugs or other chemicals, or manipulations of physical condition to test the resiliency of the animal. If eggs are hatched, the offspring must be reared normally. Otherwise, all embryos must be destroyed by freezing before 85% of normal incubation.
5. Cells and animal parts (including organs, tissues, plasma or serum) purchased or acquired from biological supply houses or research facilities may be used in science fair projects. Evidence of the source of the materials (e.g. bill of sale) must be available at the display.
6. The acquisition of animal parts should involve either the services of biological supply houses or research facilities, or involve salvage from sources where the animal has been killed for other legitimate purposes in a legal and humane manner. Salvage from found carcasses (e.g. road kills) is discouraged due to serious health risks. If the acquisition involves salvage from a research project at an institution, then the disposition to the science fair project must be part of the original research proposal, and the Research Committee or the Animal Care Committee of the institution involved must have approved the disposition. Reference to the original project should be made on the science project. If the acquisition involves salvage from the food industry, then the source must be acknowledged. If the acquisition involves hunting, fishing or trapping, then those activities must be done in accordance with prevailing regulations, and precautions must be taken to ensure the safety of the student(s). The taking of animals other than for food, without explicit approval, can constitute cruelty. Permits for research are available from conservation authorities.